13.02.2020

https://iopscience.iop.org/article/10.1088/1758-5090/ab7554

The feasibility of magnetic levitational bioassembly of tissue engineered constructs from living tissue spheroids in the presence of paramagnetic ions (i.e. Gd3+) was recently demonstrated. However, Gd3+ is relatively toxic at concentrations above 50 mM normally used to enable magnetic levitation with NdFeB-permanent magnets. Using a high magnetic field (a 50 mm-bore, 31 T Bitter magnet) in High Field Magnet Laboratory in Radboud University in Nijmegen, the Netherlands, we performed magnetic levitational assembly of tissue constructs from living spheroids prepared from SW1353 chondrosarcoma cell line at 0.8 mM Gd3+ containing salt gadobutrol at 19 T magnetic field. The parameters of levitation process were determined on the basis of polystyrene beads with a 170 μm-diameter. To predict the theoretical possibility of assembly, a zone of stable levitation in the horizontal and vertical area of cross sections was previously calculated. The construct from tissue spheroids partially fused after 3 hours in levitation. The analysis of viability after prolonged exposure (1 hour) to strong magnetic fields (up to 30 T) showed the absence of significant cytotoxicity or morphology changes in the tissue spheroids. High magnetic field works as a temporal and removal support or so-called "scaffield". Thus, formative biofabrication of tissue-engineered constructs from tissue spheroids in the high magnetic field is a promising research direction